1. Presence/Absence GMO Screening
GMO Screening, which usually targets a combination or promoter / terminator / marker gene DNA sequences is the most efficient and cost effective means of determining GMO presence in seed and grain lots.
2. Quantitative PCR
In some cases, on-site testing, such as lateral flow strip testing, might reveal the presence of GM but with insufficient confidence with respect to a given AP threshold (eg 0.9% w/w GMO). In this case, the sample should be submitted for quantitative real-time PCR testing to more precisely determine the % w/w amount of GMO seed / grain present.
For the seed and grain industry, sampling is inextricably interrelated with testing in achieving a particular adventitious presence (AP) threshold for GMO in a seed lot. In Australia, such thresholds do vary and might be determined by government regulations or market requirements.
The sub-sampling/PCR approach is the most precise means by which to monitor AP levels of GMO in seed lots. Sampling theory is used to determine the minimum number of seeds required to sample GM at a particular AP threshold and at a required level of confidence.
4. Tests Available
Our service is focused on Australian production. To this end, we are licensed by the relevant technology providers to undertake event-specific PCR testing of all GM traits approved for commercial release in Australia.
AgriGen also offers an extensive range of GMO screening tests. These tests are used to widely to monitor AP during QA or to verify the GM status of imported conventional seed.
In addition to PCR testing, we also conduct both ELISA testing and lateral flow strip testing.
5. Support and Further Training
AgriGen is able to provide assistance either informally or via workshops on technical issues relating to the use of on-site tests in monitoring AP.
Workshops are intended to be more hands-on and practical, but with sufficient theoretical background to provide participants with a good knowledge of GMO’s and GMO testing. In particular, critical aspects such as sample preparation and the use of controls to assist in results interpretation are emphasised.
If you need to discuss these tests in more detail, please contact AgriGen at firstname.lastname@example.org or by phoning +61 (0)2 8856 0988